TBC1 Domain Family, Member 4 (TBC1D4) antibody

Antigen: TBC1 Domain Family, Member 4 (TBC1D4)

Synonyms: AS160, DKFZp779C0666, As160, AV295684, KIAA0603, A930035N22, 5930406J04Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN190878

Additional Information

Alternative name: AS160 / TBC1D4

Gene ID: 9882

Immunogen: Peptide with sequence C-DDPEKIEERKKSK, from the internal region of the protein sequence according to NP_055647.2

Description: All names and symbols: TBC1D4, AS160, TBC1 domain family, member 4 , RP11-159J2.1, DKFZp779C0666 , Acrg embryonic lethality (mouse) minimal region ortholog, OTTHUMP00000040818, TBC (Tre-2, BUB2, CDC16) domain-containing protein, KIAA0603. Official Symbol: TBC1D4. RefSeq number(s): NP_055647.2.

Specificity: Expected from sequence similarity: Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 150kDa band observed in lysates of cell lines Daudi and MOLT4 (calculated MW of 147kDa according to NP_055647.2). Recommended concentration: 0.1-0.3µg/ml. Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Karlsson, Zierath, Kane et al.: "Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects." in: Diabetes, Vol. 54, Issue 6, pp. 1692-7, 2005 (PubMed).