APPL1 antibody (Internal Region)

Catalog No. ABIN190879

This Goat Polyclonal antibody specifically detects APPL1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for APPL1 antibody (Internal Region) (ABIN190879)

Target: APPL1 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APPL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-APPL1 Antibody

Binding Specificity: Internal Region

Purpose: APPL1

Sequence: NRYSRLSKKR ENDK

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NRYSRLSKKRENDK, from the internal region of the protein sequence according to NP_036228.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in Rat Aortic Smooth Skeletal Muscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7 kDa according to NP_036228.1. The blot was co-labeled wit

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for APPL1

Target: APPL1 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1))

Alternative Name: APPL1

Background: APPL1, DIP13alpha , adaptor protein containing pH domain, PTB domain and leucine zipper motif, signaling adaptor protein DIP13alpha

Gene ID: 26060, 72993

NCBI Accession: NP_036228