Luteinizing Hormone/choriogonadotropin Receptor (LHCGR) antibody

Antigen: Luteinizing Hormone/choriogonadotropin Receptor (LHCGR)

Synonyms: HHG, LHR, LCGR, LGR2, ULG5, LHRHR, LSH-R, LH/CGR, LH/CG-R, FLJ41504, Lhr, LH-R, Gpcr19-rs1, LH receptor, LHCGR, lhcgr-A, lhr, GTHR-II, zfGTHR-II, LOC100222883, lh-r, LOC100305044

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190901

Additional Information

Alternative name: LHCGR

Gene ID: 3973

Immunogen: Peptide with sequence CQGTALLDKTRYTE, from the C Terminus of the protein sequence according to NP_000224.2.

Format: Affinity-purified

Description: All names and symbols: LHCGR, luteinizing hormone/choriogonadotropin receptor , LCGR, LGR2, LHR, hLHR , luteinizing hormone receptor, lutropin/choriogonadotropin receptor, LH receptor, CG receptor. Official Symbol: LHCGR. RefSeq number(s): NP_000224.2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 35kDa band in lysates of Human Brain, Placenta and Prostate and of cell lines Daudi, HeLa and K562 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.6kDa according to NP_000224.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Bukovsky, Indrapichate, Fujiwara et al.: "Multiple luteinizing hormone receptor (LHR) protein variants, interspecies reactivity of anti-LHR mAb clone 3B5, subcellular localization of LHR in human placenta, pelvic floor and brain, and possible role for LHR in the development of abnormal pregnancy," in: Reproductive biology and endocrinology : RB&E, Vol. 1, pp. 46, 2004 (PubMed).