LHCGR antibody (N-Term)

Catalog No. ABIN3042675

Product Details anti-LHCGR Antibody

Target: LHCGR (Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR))

Binding Specificity: AA 127-143, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LHCGR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Lutropin-choriogonadotropic hormone receptor(LHCGR) detection. Tested with WB, IHC-P in Human,Rat.

Sequence: YLSICNTGIR KFPDVTK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Lutropin-choriogonadotropic hormone receptor(LHCGR) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: luteinizing hormone/choriogonadotropin receptor
Protein Name: Lutropin-choriogonadotropic hormone receptor(LH/CG-R)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human hCG receptor(127-143aa YLSICNTGIRKFPDVTK), different from the relative rat sequence by two amino acids, mouse sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human , Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-LHCGR antibody (ABIN3042675)

Wu, Shi, Gong, Yu, Chen, Wang, Sun: "Evaluation of follicular synchronization caused by estrogen administration and its reproductive outcome." in: PLoS ONE, Vol. 10, Issue 5, pp. e0127595, (2016) (PubMed).

Target Details for LHCGR

Target: LHCGR (Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR))

Alternative Name: LHCGR (LHCGR Products)

Synonyms: HHG antibody, LCGR antibody, LGR2 antibody, LH/CG-R antibody, LH/CGR antibody, LHR antibody, LHRHR antibody, LSH-R antibody, ULG5 antibody, Gpcr19-rs1 antibody, LH-R antibody, Lhr antibody, LSHR antibody, lhr antibody, GTHR-II antibody, zfGTHR-II antibody, LHCGR antibody, LOC100222883 antibody, lh-r antibody, LOC100305044 antibody, luteinizing hormone/choriogonadotropin receptor antibody, luteinizing hormone/choriogonadotropin receptor L homeolog antibody, luteinizing hormone receptor antibody, LHCGR antibody, Lhcgr antibody, lhcgr antibody, lhcgr.L antibody, lhr antibody, LOC100305044 antibody

Background: The luteinizing hormone/choriogonadotropin receptor is a member of a subfamily of G protein-coupled receptors(GPCR) characterized by the presence of a large N-terminal extracellular domain containing several leucine-rich repeats(LRR). This glycoprotein hormone receptor family has been named the LRR-containing GPCR(LGR) family. The LHCGR gene contains 11 exons and spans approximately 80 kb and it is mapped to chromosome 2p21. In the ovary, theca, stromal, late-stage(luteinizing) granulosa, and luteal cells contain LHCGR. In the testes, Leydig cells contain LHCRG.

Synonyms: Gonadotropin receptor antibody|Gpcr19-rs1 antibody|GTHR-II antibody|HHG antibody|LCGR antibody|LGR2 antibody|LH-R antibody|LH/CG R antibody|LH/CG-R antibody|LH/CGR antibody|LHCGR antibody|LHR antibody|LHRHR antibody|LSH R antibody|LSH-R antibody|LSHR_HUMAN antibody|Luteinizing hormone receptor antibody|Luteinizing hormone/choriogonadotropin receptor antibody|Lutropin choriogonadotropic hormone receptor antibody|Lutropin choriogonadotropic receptor antibody|Lutropin-choriogonadotropic hormone receptor antibody|ULG5 antibody

UniProt: P22888

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, cAMP Metabolic Process, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling