Disrupted in Schizophrenia 1 (DISC1) antibody

Antigen: Disrupted in Schizophrenia 1 (DISC1)

Synonyms: SCZD9, C1orf136, FLJ13381, FLJ21640, FLJ25311, FLJ41105, KIAA0457, DISC1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190904

Additional Information

Alternative name: DISC1

Gene ID: 27185

Immunogen: C-HSEDKRKTPLKVLE

Format: Affinity-purified

Description: All names and symbols: DISC1, disrupted in schizophrenia 1 , RP4-730B13.1, KIAA0457. Official Symbol: DISC1. RefSeq number(s): NP_001012975.1, NP_061132.2

Specificity: This antibody is expected to recognise isoforms L ( NP_061132.2) and Lv (NP_001012975.1) only. Human 854 aa, 832 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 100kDa band observed in human brain (cerebellum, hippocampus and frontal cortex) lysates (calculated MW of 93.6kDa according to NP_001012975.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurology, Alzheimer's Disease

Restrictions: For Research Use only

Images

ABIN190904 (0.1µg/ml) staining of human brain (hippocampus) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Millar, Pickard, Mackie et al.: "DISC1 and PDE4B are interacting genetic factors in schizophrenia that regulate cAMP signaling." in: Science (New York, N.Y.), Vol. 310, Issue 5751, pp. 1187-91, 2005 (PubMed).