Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H) antibody
| Antigen | Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H) |
| Synonyms |
CDC68, FACTP140, FLJ10857, FLJ14010, FLJ34357, SPT16/CDC68, CG1828, DmelCG1828, DRE4/dSPT16, dspt16, dSpt16, dSPT16, l(3)62Bg, l(3)dre4, spt16, Spt16, Cdc68, Fact140, gtc102, global transcription fact ...
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| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Human |
| Conjugate |
Alternatives Un-conjugated |
| Application |
Alternatives ELISA, Western Blotting (WB) |
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1 reference available |
| Catalog no. | ABIN190913 |
| Quantity | 100 ug |
| Price | 374.27 $ Plus shipping costs $45.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | FACT / SUPT16H |
| Gene ID | 11198 |
| Immunogen | C-DLTNKEGKKPEEK |
| Description | All names and symbols: SUPT16H, FACT, suppressor of Ty 16 homolog (S. cerevisiae), HGNC:11465, CDC68, FACTP140, FLJ10857, FLJ14010, SPT16/CDC68, chromatin-specific transcription elongation factor large subunit. Official Symbol: SUPT16H. RefSeq number(s): NP_009123.1 |
| Specificity | Human |
| Synonyms | CDC68, FACTP140, FLJ10857, FLJ14010, FLJ34357, SPT16/CDC68, CG1828, DmelCG1828, DRE4/dSPT16, dspt16, dSpt16, dSPT16, l(3)62Bg, l(3)dre4, spt16, Spt16, Cdc68, Fact140, gtc102, global transcription factor C, SPT16, T12H20.3, T12H20_3, SSF1 |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 120kDa according to NP_009123.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use only |
Images
| ABIN190913 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Publications
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Belotserkovskaya, Oh, Bondarenko et al.: "FACT facilitates transcription-dependent nucleosome alteration." in: Science (New York, N.Y.), Vol. 301, Issue 5636, pp. 1090-3, 2003 (PubMed).
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Alternatives
Alternatives for antigen "Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H)", type "Antibodies"
| Hosts | Rabbit (34), Goat (2), Mouse (2) |
| Reactivities | Human (35), Mouse (Murine) (24), Cow (Bovine) (17), Dog (Canine) (17), Rat (Rattus) (17), Zebrafish (Danio rerio) (17) |
| Applications | Western Blotting (WB) (21), Immunofluorescence (IF) (14), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1) |
| Conjugates | Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1) |
| Epitopes | Internal Region (2), N-Term (2), AA 321-640 (1), AA 802-982 (1), C-Term (1), Center (1), Internal Region,AA 369-399 (1) |
