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Collagen, Type VII (COL7) antibody

Details for Product No. ABIN190915, Supplier: Log in to see
Antigen
  • COL7A1
Reactivity
Rat (Rattus)
41
25
17
2
2
2
1
1
1
1
1
Host
Goat
24
17
12
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
23
22
15
13
13
11
5
4
4
3
1
1
1
1
1
Supplier
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Immunogen C-TVQYSDDPRTEF
Blocking Peptide Blocking peptide for this product available: ABIN370008
Specificity This product does not work on cryostat sections of Normal Human Skin. Human, Mouse, Rat, Dog
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Collagen, type VII (COL7 Antibody Abstract)
Background All names and symbols: Collagen, type VII, collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive), COL7A1, HGNC:2214, EBD1, EBDCT, EBR1, LC collagen, alpha 1 type VII collagen, collagen VII, alpha-1 polypeptide, long chain collagen. Official Symbol: COL7A1. RefSeq number(s): NP_000085.1
Gene ID 1294, 12836
Pathways
Application Notes Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 250kDa band observed in rat testis lysates (calculated MW of 159kDa according to Rat NP_001100328.1). Recommended concentration: 0.1-1.5µg/ml. ABIN190915 did not work in IF on human skin.
Restrictions For Research Use only
Format Liquid
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-Collagen, Type VII (COL7) antibody (ABIN190915) ABIN190915 (0.5µg/ml) staining of rat testis lysate (35µg protein in RIPA buffer). Pr...
Background publications Ortiz-Urda, Garcia, Green et al.: "Type VII collagen is required for Ras-driven human epidermal tumorigenesis." in: Science (New York, N.Y.), Vol. 307, Issue 5716, pp. 1773-6, 2005 (PubMed).