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Collagen, Type VII (COL7) antibody
| Antigen | Collagen, Type VII (COL7) |
| Synonyms | EBD1, EBR1, EBDCT, COL7A1 |
| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Rat (Rattus) |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN190915 |
| Quantity | 100 ug |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | Collagen, type VII |
| Gene ID | 1294, 12836 (mouse) |
| Immunogen | C-TVQYSDDPRTEF |
| Format | Affinity-purified |
| Description | All names and symbols: Collagen, type VII, collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive), COL7A1, HGNC:2214, EBD1, EBDCT, EBR1, LC collagen, alpha 1 type VII collagen, collagen VII, alpha-1 polypeptide, long chain collagen. Official Symbol: COL7A1. RefSeq number(s): NP_000085.1 |
| Specificity | This product does not work on cryostat sections of Normal Human Skin. Human, Mouse, Rat, Dog |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 250kDa band observed in rat testis lysates (calculated MW of 159kDa according to Rat NP_001100328.1). Recommended concentration: 0.1-1.5µg/ml. ABIN190915 did not work in IF on human skin. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use only |
Images
| ABIN190915 (0.5µg/ml) staining of rat testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Publications
| Publications |
Ortiz-Urda, Garcia, Green et al.: "Type VII collagen is required for Ras-driven human epidermal tumorigenesis." in: Science (New York, N.Y.), Vol. 307, Issue 5716, pp. 1773-6, 2005 (PubMed).
|
Alternatives
Alternatives for antigen "Collagen, Type VII (COL7)", type "Antibodies"
| Hosts | Rabbit (25), Mouse (15), Goat (2) |
| Reactivities | Human (34), Mouse (Murine) (25), Cow (Bovine) (21), Dog (Canine) (21), Rat (Rattus) (21), Pig (Porcine) (18), Rabbit (17), Chicken (4), Horse (Equine) (2), Marmoset (2), Goat (1), Guinea Pig (1), Monkey (1), Sheep (Ovine) (1) |
| Applications | Immunofluorescence (IF) (28), Flow Cytometry (FACS) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (12), Western Blotting (WB) (7), ELISA (6), Immunoelectron Microscopy (IEM) (6), Immunohistochemistry (IHC) (6), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
