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Enolase 2 (Gamma, Neuronal) (ENO2) antibody

Antigen

Enolase 2 (Gamma, Neuronal) (ENO2)

Synonyms ENO2, DKFZp459B1817, NSE, Eno-2, AI837106, D6Ertd375e, RNEN3, Lrpb7
Clonality Monoclonal (1C1)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Application
Alternatives Enzyme Immunoassay (EIA), ELISA (Detection)
Catalog no. ABIN191284
Quantity 1 mg
Price 442.54 $   Plus shipping costs $35.00
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Additional Information

Alternative name Neuron specific enolase
Immunogen Hybridoma Clone s have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.
Isotype IgG2a  (Matching secondary antibodies)
Clone 1C1
Specificity Human NSE IgG2a for MAbs 5E2, 1C1, 5A4, H11 and 2F1 IgG2b for MAb 5G10

Application Details

Application Notes MAbs 5G10, 5A4 and 5E2 react with 45 kD band corresponding to NSE in immunoblotting of human brain extracts. MAbs may be used for immunohistochemistry on formalin-fixed and paraffin-embedded sections. Recommended dilution is 1:100 - 1:300. Optimal dilution must be determined by experimentation. All MAbs work in ELISA. Recommended pairs for sandwich immunoassay (coating – conjugate): 5G10 – 1C1 5E2 – 1C1 1C1 – 5E2 5A4 – 1C1 H11 – 2F1
Buffer PBS, pH 7.4, 0.1 % sodium azide (NaN3) Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative
Storage + 4 °C
Research Area Cell/Tissue Markers, Cancer
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Enolase 2 (Gamma, Neuronal) (ENO2)", type "Antibodies"
Hosts Mouse (59), Rabbit (23), Chicken (1), Sheep (1)
Reactivities Human (69), Guinea Pig (7), Mouse (Murine) (7), Rat (Rattus) (7), Chicken (2), Dog (Canine) (2), Cow (Bovine) (1), Pig (Porcine) (1)
Applications Western Blotting (WB) (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (34), Enzyme Immunoassay (EIA) (27), ELISA (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunoprecipitation (IP) (9), Immunohistochemistry (IHC) (8), Immunofluorescence (IF) (7), ELISA (Capture) (4), ELISA (Detection) (4), Immunocytochemistry (ICC) (1)
Epitopes pTyr236 (1)