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Antibodies
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anti-Enolase 2 (Gamma, Neuronal) (ENO2) antibody
Enolase 2 (Gamma, Neuronal) (ENO2) antibody
| Antigen |
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| Synonyms |
ENO2, DKFZp459B1817, NSE, Eno-2, AI837106, D6Ertd375e, RNEN3, Lrpb7 |
| Clonality |
Monoclonal (1C1) |
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Host
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Reactivity
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Application
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Western Blotting (WB) (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (34), Enzyme Immunoassay (EIA) (27), ELISA (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunoprecipitation (IP) (9), Immunohistochemistry (IHC) (8), Immunofluorescence (IF) (7), ELISA (Capture) (4), ELISA (Detection) (4), Immunocytochemistry (ICC) (1)
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| Catalog no. |
ABIN191284 |
| Quantity |
1 mg |
| Price |
442.54 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Neuron specific enolase
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Immunogen
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Hybridoma Clone s have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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1C1
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Specificity
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Human NSE IgG2a for MAbs 5E2, 1C1, 5A4, H11 and 2F1 IgG2b for MAb 5G10
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Application Notes
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MAbs 5G10, 5A4 and 5E2 react with 45 kD band corresponding to NSE in immunoblotting of human brain extracts. MAbs may be used for immunohistochemistry on formalin-fixed and paraffin-embedded sections. Recommended dilution is 1:100 - 1:300. Optimal dilution must be determined by experimentation. All MAbs work in ELISA. Recommended pairs for sandwich immunoassay (coating – conjugate): 5G10 – 1C1 5E2 – 1C1 1C1 – 5E2 5A4 – 1C1 H11 – 2F1
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Buffer
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PBS, pH 7.4, 0.1 % sodium azide (NaN3) Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative
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Storage
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+ 4 °C
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Research Area
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Cell/Tissue Markers, Cancer
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Restrictions
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For Research Use only
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Alternatives for antigen "Enolase 2 (Gamma, Neuronal) (ENO2)", type "Antibodies"
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Hosts
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Mouse (59), Rabbit (23), Chicken (1), Sheep (1)
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Reactivities
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Human (69), Guinea Pig (7), Mouse (Murine) (7), Rat (Rattus) (7), Chicken (2), Dog (Canine) (2), Cow (Bovine) (1), Pig (Porcine) (1)
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Applications
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Western Blotting (WB) (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (34), Enzyme Immunoassay (EIA) (27), ELISA (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunoprecipitation (IP) (9), Immunohistochemistry (IHC) (8), Immunofluorescence (IF) (7), ELISA (Capture) (4), ELISA (Detection) (4), Immunocytochemistry (ICC) (1)
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Epitopes
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pTyr236 (1)
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