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Antibodies
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anti-S100 Calcium Binding Protein A1 (S100A1) (Ab) antibody
S100 Calcium Binding Protein A1 (S100A1) (Ab) antibody
| Antigen |
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| Synonyms |
S100, S100A, S100-alpha, S100a1, S100A1, MGC159706, DKFZp468K0511 |
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Epitope
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| Clonality |
Monoclonal (3B10) |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), ELISA (Capture), Western Blotting (WB)
Western Blotting (WB) (18), Immunofluorescence (IF) (16), Flow Cytometry (FACS) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), ELISA (9), Enzyme Immunoassay (EIA) (8), Immunoprecipitation (IP) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (IHC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN191327 |
| Quantity |
1 mg |
| Price |
487.18 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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S100A1 (ab)
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Immunogen
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Hybridoma Clone s have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human brain S-100 protein.
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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3B10
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Specificity
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MAbs 8B10, 6G1 and 4B3 are specific to S-100ββ and S-100αβ. MAb 3B10 is specific to S-100αβ. IgG1 for MAbs 8B10 and 6G1 IgG2a for MAbs 3B10 and 4B3
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Application Notes
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S-100 immunoassay and immunoaffinity purification (except 4B3). All MAbs are working in Western blotting. Recommended pairs for sandwich immunoassay (coating – conjugate): 8B10 – 6G1 3B10 – 6G1 MAbs 8B10, 3B10 and especially 6G1 are sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5 mM CaCl2 in the assay buffers.
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Buffer
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PBS, pH 7.4, 0.1 % sodium azide (NaN3) Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative
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Storage
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+ 4 °C
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Restrictions
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For Research Use only
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Alternatives for antigen "S100 Calcium Binding Protein A1 (S100A1)", type "Antibodies"
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Hosts
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Rabbit (25), Mouse (8), Sheep (2)
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Reactivities
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Human (26), Cow (Bovine) (21), Mouse (Murine) (18), Rat (Rattus) (18), Chicken (15), Dog (Canine) (15), Horse (Equine) (15), Pig (Porcine) (2), Mammalian (1)
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Applications
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Western Blotting (WB) (18), Immunofluorescence (IF) (16), Flow Cytometry (FACS) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), ELISA (9), Enzyme Immunoassay (EIA) (8), Immunoprecipitation (IP) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (IHC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (2)
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