FASL antibody (C-Term)

Catalog No. ABIN191900

The Rabbit Polyclonal anti-FASL antibody is suitable to detect FASL in samples from Mouse and Rat. It has been validated for WB.

Quick Overview for FASL antibody (C-Term) (ABIN191900)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FASL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FASL Antibody

Binding Specificity: C-Term

Specificity: Detects ~31 kDa FasL. A ~70 kDa band, possible represents the FasL and Fas complex, can also be detected.

Purification: Affinity chromatography

Immunogen: Synthetic peptide mapping to the C-terminus of rat FasL

Application Details

Application Notes: Western blotting (0.5-4 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store in aliquots at -20 °C.

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: CD178 / Fas Ligand

Background: Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6

Gene ID: 14103

NCBI Accession: NP_034307

UniProt: P41047

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity