1,25 Dihydroxy Vitamin D3 (DHVD3) antibody

Antigen: 1,25 Dihydroxy Vitamin D3 (DHVD3)

Clonality: Monoclonal (ID125A)

Host: Mouse

Application: Enzyme Immunoassay (EIA)

Catalog no.: ABIN191979

Additional Information

Characteristics: Synonyms: Calcitriol

Alternative name: 1,25-Dihydroxy-Vitamin D3

Immunogen: 1,25(OH)2 D3 3-Hemisuccinat-BSA

Isotype: IgG1  (Matching secondary antibodies)

Clone: ID125A

Specificity: This antibody detects 1,25-Dihydroxy-Vitamin D3. Cross Reactivity (after extraction with ELISA K2112) vs. : 1,25(OH)2D3 (Calcitriol): 100% (100%)1,25(OH)2D2 (Ergocalcitriol): 100% (46%)24,25(OH)2D3 (24,25-dihydroxycholecaciferol): 20. 5% (< 0. 1%)25-(OH)D3 (Calcidiol): 4. 4% (< 0. 01%)25-(OH)D2 (Ergocalcidiol): 2. 3% (< 0. 1%)D3 (Cholecalciferol): 0. 3% (< 0. 01%)D2 (Ergocalciferol) 1. 0% (< 0. 01%)

Application Details

Application Notes: ELISA (1/25000)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: 50 mM TRIS pH 7.4Reconstitution: Restore in aqua bidest to 1 mg/ml.

Storage: Store lyophilized at 2-8°C and reconstituted at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Research Area: Vitamins

Restrictions: For Research Use only