CD235ab antibody (N-Term) (FITC)

Catalog No. ABIN192327

The FITC-conjugated Mouse Monoclonal anti-CD235ab antibody (Clone HIR2) (ABIN192327) specifically detects CD235ab in FACS. The antibody is reactive with Human samples.

Quick Overview for CD235ab antibody (N-Term) (FITC) (ABIN192327)

Target: CD235ab (CD235a + CD235b) (Glycophorin A + B (CD235a + CD235b))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235ab antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: HIR2

Product Details anti-CD235ab Antibody (FITC)

Binding Specificity: N-Term

Purpose: Anti-Hu CD235ab FITC

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody HIR2 recognizes an epitope within the N-terminal (extracellular) portion of CD235a (glycophorin A) and CD235b (glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Synthetic peptide (Human, N-terminal)

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for CD235ab

Target: CD235ab (CD235a + CD235b) (Glycophorin A + B (CD235a + CD235b))

Alternative Name: CD235ab

Background: CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.,Glycophorin A/B, GYPA/B, GPA/B, PAS-2/3, Sialoglycoprotein alpha/beta, GPSAT, GPErik, MSN blood group

Gene ID: 35