PHKB antibody

Catalog No. ABIN1944863

There are 3+ publications for this product available. The Rabbit Polyclonal anti-PHKB antibody is suitable to detect PHKB in samples from Human and Mouse. It has been validated for WB.

Quick Overview for PHKB antibody (ABIN1944863)

Target: PHKB (Phosphorylase Kinase, beta (PHKB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHKB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PHKB Antibody

Isotype: IgG

Application Details

Application Notes: WB: 1:500- 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C,-20 °C

References for anti-PHKB antibody (ABIN1944863)

Oppermann, Gnad, Olsen, Hornberger, Greff, Kéri, Mann, Daub: "Large-scale proteomics analysis of the human kinome." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1751-64, (2009) (PubMed).

Beausoleil, Villén, Gerber, Rush, Gygi: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, (2006) (PubMed).

Wüllrich-Schmoll, Kilimann: "Structure of the human gene encoding the phosphorylase kinase beta subunit (PHKB)." in: European journal of biochemistry / FEBS, Vol. 238, Issue 2, pp. 374-80, (1996) (PubMed).

Target Details for PHKB

Target: PHKB (Phosphorylase Kinase, beta (PHKB))

Alternative Name: PHKB

Background: Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.

Molecular Weight: 124884 Da

Gene ID: 5257

UniProt: Q93100

Pathways: Cellular Glucan Metabolic Process