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LIM Domain Kinase 2 (LIMK2) (pThr505) antibody

Details for Product No. ABIN196832
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Antigen
Synonyms xlimk2, zgc:91990, wu:fa97c09, wu:fk73e11, LIMK2, Limk2b, Link2, LIMK, 9430059K01, A930024P04Rik, C85310, Limk2a
Epitope
pThr505
(22), (11), (10), (8), (6), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(100), (62), (62)
Host
Rabbit
(92), (5), (3)
Clonality
Polyclonal
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(91), (46), (46), (26), (9), (9), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN196832
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).
Specificity This antibody detects endogenous levels of LIMK2 only when phosphorylated at Threonine 505.
Purification Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name LIMK2
Background LIM kinases (LIM kinase 1 and LIM kinase 2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs in their N terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIM kinase 1 and LIM kinase 2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N terminus of Cofilin.
Alternate names: EC=2.7.11.1, LIM domain kinase 2, LIMK-2, LIMK2
Gene ID 3985
NCBI Accession NP_005560.1
UniProt P53671
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Supplier Images
anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody
anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody (2) anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody (Image 2)
anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody (3) anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody (Image 3)
Background publications Edwards, Sanders, Bokoch et al.: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, 1999 (PubMed).

Ohashi, Nagata, Maekawa et al.: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Validation Images
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