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LIM Domain Kinase 2 (LIMK2) (pThr505) antibody

Details for Product No. ABIN196832, Supplier: Log in to see
Antigen
  • xlimk2
  • zgc:91990
  • wu:fa97c09
  • wu:fk73e11
  • LIMK2
  • Limk2b
  • Link2
  • LIMK
  • 9430059K01
  • A930024P04Rik
  • C85310
  • Limk2a
Epitope
pThr505
25
14
10
9
7
6
6
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
122
60
57
1
Host
Rabbit
110
7
3
2
Clonality
Polyclonal
Conjugate
Un-conjugated
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
105
52
44
36
10
8
2
1
1
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).
Specificity This antibody detects endogenous levels of LIMK2 only when phosphorylated at Threonine 505.
Purification Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name LIMK2 (LIMK2 Antibody Abstract)
Background LIM kinases (LIM kinase 1 and LIM kinase 2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs in their N terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIM kinase 1 and LIM kinase 2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N terminus of Cofilin.Synonyms: EC=2.7.11.1, LIM domain kinase 2, LIMK-2, LIMK2
Gene ID 3985
NCBI Accession NP_005560
UniProt P53671
Pathways
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody (ABIN196832) anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody
 image for anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody (ABIN196832) anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody (Image 2)
 image for anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody (ABIN196832) anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibody (Image 3)
Background publications Ohashi, Nagata, Maekawa et al.: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Edwards, Sanders, Bokoch et al.: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, 1999 (PubMed).