ZAP70 antibody (pTyr493)

Catalog No. ABIN196886

Product Details anti-ZAP70 Antibody

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Binding Specificity: pTyr493

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyrosine 493.

Purification: Immunoaffinity chromatography using epitope-specific phosphopeptide.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human Zap-70 around the phosphorylation site of tyrosine 493 (S-Y-Yp-T-A).

Application Details

Application Notes: Western Blot 1/500-1/1000). Immunohistochemistry on Paraffin-Embedded Sections (1/50-1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70 (ZAP70 Products)

Synonyms: SRK antibody, STCD antibody, STD antibody, TZK antibody, ZAP-70 antibody, 70kDa antibody, AI327364 antibody, Srk antibody, mrtle antibody, mur antibody, zap70 antibody, MGC80670 antibody, MGC88962 antibody, ZAP70 antibody, zgc:110383 antibody, zeta chain of T-cell receptor associated protein kinase 70 antibody, putative tyrosine-protein kinase zap-70 antibody, zeta-chain (TCR) associated protein kinase antibody, zeta chain of T cell receptor associated protein kinase 70 antibody, zeta-chain (TCR) associated protein kinase 70kDa antibody, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog antibody, zeta chain of T cell receptor associated protein kinase 70kDa antibody, tyrosine-protein kinase ZAP-70 antibody, ZAP70 antibody, Smp_149470 antibody, Zap70 antibody, zap70.L antibody, zap70 antibody, LOC707555 antibody, LOC100342021 antibody, LOC100390922 antibody, LOC100542877 antibody

Background: ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70

Gene ID: 7535

NCBI Accession: NP_001070

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway