G3BP1 antibody (Ser232)

Catalog No. ABIN197272

This Rabbit Polyclonal antibody specifically detects G3BP1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for G3BP1 antibody (Ser232) (ABIN197272)

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G3BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-G3BP1 Antibody

Binding Specificity: Ser232

Specificity: G3BP-1 antibody detects endogenous levels of total G3BP-1 protein.

Purification: Affinity-chromatography using epitope-specific immunogen

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 (S-S-SP-P-A).

Application Details

Application Notes: Western Blot: 1: 500approx. 1000. Immunohistochemsistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for G3BP1

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Alternative Name: G3BP1 / G3BP

Background: G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.Synonyms: ATP-dependent DNA helicase VIII, G3BP-1, GAP SH3 domain-binding protein 1, Ras GTPase-activating protein-binding protein 1, hDH VIII

Gene ID: 10146

NCBI Accession: NP_005745

UniProt: Q13283

Pathways: SARS-CoV-2 Protein Interactome