Endoglin antibody (PE)

Catalog No. ABIN1981859

Product Details anti-Endoglin Antibody (PE)

Target: Endoglin (ENG)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Endoglin antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The rat monoclonal antibody MJ7/18 reacts with an extracellular epitope of CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow.

Cross-Reactivity (Details): Mouse

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Inflamed mouse skin

Clone: MJ7-18

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-Endoglin antibody (PE) (ABIN1981859)

Dassler, Roohi, Lohrke, Ide, Remmele, Hütter, Pietsch, Pison, Schütz: "Current limitations of molecular magnetic resonance imaging for tumors as evaluated with high-relaxivity CD105-specific iron oxide nanoparticles." in: Investigative radiology, Vol. 47, Issue 7, pp. 383-91, (2012) (PubMed).

Rosu-Myles, She, Fair, Muradia, Mehic, Menendez, Prasad, Cyr: "Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells." in: PLoS ONE, Vol. 7, Issue 6, pp. e38954, (2012) (PubMed).

Fonsatti, Nicolay, Altomonte, Covre, Maio: "Targeting cancer vasculature via endoglin/CD105: a novel antibody-based diagnostic and therapeutic strategy in solid tumours." in: Cardiovascular research, Vol. 86, Issue 1, pp. 12-9, (2010) (PubMed).

Warrington, Hillarby, Li, Letarte, Kumar: "Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells." in: Anticancer research, Vol. 25, Issue 3B, pp. 1851-64, (2005) (PubMed).

Herouet, Cottin, LeClaire, Enk, Rousset: "Contact sensitizers specifically increase MHC class II expression on murine immature dendritic cells." in: In vitro & molecular toxicology, Vol. 13, Issue 2, pp. 113-23, (2001) (PubMed).

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105 (ENG Products)

Background: Endoglin,CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.,Endoglin, END, ENG

Gene ID: 13805

UniProt: Q63961