TJP1 antibody (Middle Region)

Catalog No. ABIN199816

This Rabbit Polyclonal antibody specifically detects TJP1 in WB, IHC, IF and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for TJP1 antibody (Middle Region) (ABIN199816)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TJP1 Antibody

Binding Specificity: Middle Region

Specificity: Recognizes human ~225kD ZO-1 protein. Bands of unknown origin may be observed at lower molecular weights in some sample lysates. These bands may represent breakdown products. Reactivity has been confirmed with dog MDCK-II, human A431 and Caco-2, rat Rat-1 and NRK-52E cell lysates by Western Blot and with MDCK-II, Caco-2, Rat-1 and NRK-52E cells by Immunofluorescence. Reactivity has also been confirmed with frozen mouse liver, blood vessel (in heart) and mossy fiber terminal (in brain) tissues by Immunohistochemistry and Immunofluorescence.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the mid region of human ZO-1, which differs from dog, mouse, and rat by one conservative amino acid replacement.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (2.5 μg/mL), IHC, IHC-Fr, WB (1 - 3 μg/mL)

Usage: Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Western Blot: 1-3 μg/mL. Immunofluorescence: 2.5 μg/mL. Immunohistochemistry (frozen): 2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: TJP1 / ZO-1

Background: Name/Gene ID: TJP1
Family: Occludin

Synonyms: TJP1, ZO-1, Zona occludens protein 1, Zona occludens 1, Zonula occludens protein 1, Tight junction protein 1, ZO1, Tight junction protein ZO-1, Zonula occludens 1 protein

Gene ID: 7082

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization