CDK1 antibody

Catalog No. ABIN199903

This Mouse Monoclonal antibody specifically detects CDK1 in WB, IHC, IP and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CDK1 antibody (ABIN199903)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: POH-1

Product Details anti-CDK1 Antibody

Specificity: Recombinant human p34 cdc2 fusion protein.

Purification: Protein A purified

Immunogen: Recombinant human CDK1.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: IHC, IHC-P, IP, WB

Usage: This antibody is suitable for immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1 % saponin treatment or other permeabilization method.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.5 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDK1 / CDC2

Background: Name/Gene ID: CDK1
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK1, CDC28A, CDC2, CDKN1, Cyclin-dependent kinase 1, p34 protein kinase, p34CDC2, CDC2a, Cell cycle controller CDC2, Cell division protein kinase 1, PSTAIR

Gene ID: 983

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA