IRS1 antibody (pSer307)

Catalog No. ABIN199910

The Rabbit Polyclonal anti-IRS1 antibody is suitable to detect IRS1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for IRS1 antibody (pSer307) (ABIN199910)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IRS1 Antibody

Binding Specificity: pSer307

Specificity: Amino acids 298-316 of human IRS1 protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human IRS1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:10000), WB (1:250 - 1:1500)

Usage: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 180 kD in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS307 of IRS1 protein.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS1 / IRS-1

Background: Name/Gene ID: IRS1

Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1

Gene ID: 3667

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process