PIK3R1 antibody

Catalog No. ABIN201416

The Mouse Monoclonal anti-PIK3R1 antibody is suitable to detect PIK3R1 in samples from Human, Mouse and Rat. It has been validated for WB, IP and ICC.

Quick Overview for PIK3R1 antibody (ABIN201416)

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIK3R1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PIK3R1 Antibody

Specificity: Recognizes human p85 alpha subunit of PI 3- Kinase. Does not recognize p85 beta subunit. Species cross-reactivity: mouse and rat.

Purification: Protein A purified

Immunogen: Recombinant human p85 alpha PI 3- Kinase expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ICC, IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot Analysis: 0.5-2 μg/mL detected PI 3-Kinase in RIPA lysates from A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PI 3- Kinase (0.5 mg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: Use 10 μg/mL. Immunoprecipitation: 4 μg.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PIK3R1

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Alternative Name: PIK3R1 / p85 Alpha

Background: Name/Gene ID: PIK3R1
Family: Non-Protein Kinase

Synonyms: PIK3R1, p85, p85 Alpha, p85-ALPHA, PI3K regulatory subunit alpha, PI3-kinase subunit p85-alpha, GRB1

Gene ID: 5295

UniProt: P27986

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect