C3 antibody

Catalog No. ABIN204135

The Mouse Monoclonal anti-C3 antibody (ABIN204135) specifically detects C3 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for C3 antibody (ABIN204135)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-C3 Antibody

Specificity: Selected for its ability to detect human C3a in direct ELISAs and Western Blots. In these formats, this antibody shows no cross-reactivity with rhC5a or rhC3d.

Purification: Protein G purified

Immunogen: E. coli-derived rhC3a.

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Direct ELISA. Western Blot: 1-2 μg/mL. The detection limit is ~25 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~10 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3

Background: Name/Gene ID: C3

Synonyms: C3, AHUS5, ARMD9, Complement component C3, CPAMD1, Complement C3, ASP, Complement component 3

Gene ID: 718

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling