C3 antibody (C-Term)

Catalog No. ABIN2855007

The Rabbit Polyclonal anti-C3 antibody (ABIN2855007) specifically detects C3 in WB, IP, ICC, IF, IHC (p) and ISub. The antibody is reactive with Human samples.

Quick Overview for C3 antibody (C-Term) (ABIN2855007)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunosubtraction (ISub)

Product Details anti-C3 Antibody

Binding Specificity: C-Term

Specificity: The mouse reactivity of this antibody is based on mouse IHC-P testing and may be not suitable for mouse WB assay.

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to C3 (complement component 3)
C3 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.08 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: complement C3

Background: Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.

Cellular Localization: Secreted

Molecular Weight: 187 kDa

Gene ID: 718

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling