PAPPA antibody

Catalog No. ABIN2877948

The Mouse Monoclonal anti-PAPPA antibody (Clone 1_24) (ABIN2877948) specifically detects PAPPA in IHC, WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for PAPPA antibody (ABIN2877948)

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAPPA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Clone: 1_24

Product Details anti-PAPPA Antibody

Specificity: Specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid-treated PAPP-A. The epitope is different from those of clones 6F172 and 6F174 and is located on the PAPP-A subunit of the heterotetramer. In sandwich ELISA the optimal combination was clone 6F174 as coating and biotinylated clone 6F173 as detection. This product detects only unreduced PAPP-A in Western Blot. It gives the strongest signal in Immunohistochemistry compared to clone 6F172 and clone 6F174.

Purification: Protein A/G purified

Immunogen: Purified human PAPP-A.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:8000), IHC, WB

Usage: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. ELISA: 1:8000. In sandwich ELISA the optimal combination was clone 6F174 as coating and biotinylated clone 6F173 as detection. Western Blot: Detects only unreduced PAPP-A. Immunohistochemistry: Gives the strongest signal compared to clone 6F172 and clone 6F174.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Alternative Name: PAPPA / PAPP-A

Background: Name/Gene ID: PAPPA
Subfamily: Metallopeptidase M43
Family: Protease

Synonyms: PAPPA, DIPLA1, IGF-dependent IGFBP-4 protease, PAPPA1, PAPA, PAPP-A, Pappalysin-1, ASBABP2, IGFBP-4ase

Gene ID: 5069

UniProt: Q13219