PAPPA antibody (AA 351-523)

Catalog No. ABIN7470656

This Mouse Monoclonal antibody specifically detects PAPPA in IHC. It exhibits reactivity toward Human.

Quick Overview for PAPPA antibody (AA 351-523) (ABIN7470656)

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAPPA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 780M

Product Details anti-PAPPA Antibody

Binding Specificity: AA 351-523

Purpose: PAPP-A antibody (780M)

Immunogen: Recombinant fragment (within aa 351-523) of human PAPP-A protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: IHC 1:75-1:150

Comment:

Positive Control: Placenta: Trophoplast and chorion cells should show a strong cytoplasmic papp-A staining.

Product Description: PAPP-A antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Colon: Papp-A immunostaining should be completely absent in both stromal end epithelial cells.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date: 24 months

Target Details for PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Alternative Name: PAPP-A

Background: Gene Alias: ASBABP2, Aspecific BCL2 ARE binding protein 2, Differentially placenta 1 expressed protein, DIPLA1, IGF-dependent, IGFBP4ase, Insulin-like growth factor-dependent IGF-binding protein 4 protease (IGFBP-4 protease), PAPA, PAPP A, PAPPA1, Pappalysin-1, Pregnancy Associated Plasma Protein A (PAPP-A)

UniProt: Q13219