PAPPA antibody

Catalog No. ABIN2877949

This Mouse Monoclonal antibody specifically detects PAPPA in IHC, WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PAPPA antibody (ABIN2877949)

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAPPA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Product Details anti-PAPPA Antibody

Specificity: Recognizes native Pregnancy-associated plasma protein A (human, PAPP-A) Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A. The epitope of this product is different from those of clones 6F172 and 6F173. The epitope is located on the PAPP-A subunit of the heterotetramer. Reactivity: In sandwich ELISA the optimal combination is this product used as coating antibody and biotinylated clone 6F173 used as detection antibody. This product can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. This product detects both reduced and unreduced PAPP-A in Western Blot, though reaction to unreduced PAPP-A is less than that of clones 6F172 and 6F173.

Purification: Protein A/G purified

Immunogen: Purified human PAPP-A.

Type of Immunogen: Purified protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA (1:8000), IHC, WB

Usage: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. ELISA: 1:8000. In sandwich ELISA the optimal combination is this product used as coating antibody and biotinylated clone 6F173 used as detection antibody. This product can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. Western Blot: Detects both reduced and unreduced PAPP-A in Western Blot, though reaction to unreduced PAPP-A is less than that of clones 6F172 and 6F173.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Alternative Name: PAPPA / PAPP-A

Background: Name/Gene ID: PAPPA
Subfamily: Metallopeptidase M43
Family: Protease

Synonyms: PAPPA, DIPLA1, IGF-dependent IGFBP-4 protease, PAPPA1, PAPA, PAPP-A, Pappalysin-1, ASBABP2, IGFBP-4ase

Gene ID: 5069

UniProt: Q13219