PRKAA1 antibody (AA 376-392)

Catalog No. ABIN210715

This Rabbit Polyclonal antibody specifically detects PRKAA1 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Horse, Monkey, Pig and Hamster.

Quick Overview for PRKAA1 antibody (AA 376-392) (ABIN210715)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Monkey, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PRKAA1 Antibody

Binding Specificity: AA 376-392

Specificity: Recognizes human AMPK a1, Mr 63kD. Species cross-reactivity: rat and mouse.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Pig (100%) Opossum, Turkey, Chicken, Platypus (94%) Xenopus, Zebrafish (88%) Pufferfish (82%).

Purification: Protein A purified

Immunogen: Synthetic peptide of human AMP-activated Protein Kinase a1 corresponding to aa 376-392 (C-ARHTLDELNPQKSKHQG-COOH) (KLH coupled). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Pig (100%), Opossum, Turkey, Chicken, Platypus (94%), Xenopus, Zebrafish (88%), Pufferfish (82%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: PRKAA1 / AMPK Alpha 1

Background: Name/Gene ID: PRKAA1
Subfamily: AMPK/SNF1
Family: Protein Kinase

Synonyms: PRKAA1, ACACA kinase, AMPK alpha 1, AMPK alpha-1 chain, Acetyl-CoA carboxylase kinase, AMPKa1, AMPK subunit alpha-1, AMPK, AMPK1, Tau-protein kinase PRKAA1, HMGCR kinase

Gene ID: 5562

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect