Caspase 3 antibody

Catalog No. ABIN210737

The Rabbit Polyclonal anti-Caspase 3 antibody is suitable to detect Caspase 3 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for Caspase 3 antibody (ABIN210737)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 3 Antibody

Specificity: Recognizes full-length Caspase 3 (Yama/Apopain) at 32kD and proteolytic fragments as demonstrated after in vitro cleavage of recombinant Caspase 3. Cross-reacts with human, mouse and rat.

Purification: Protein A purified

Immunogen: Human full-length Caspase 3 fusion protein containing a histidine-6 tag.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.5-2 μg/mL (will detect the 32kD precursor of Caspase 3 in RIPA lysates from non-stimulated human A431 cells, previous lots detected Caspase 3 in RIPA lysates of mouse 3T3 and rat PC12 cells). Use 20 μg per lane for minigels. Positive Antigen Control: non-stimulated A431 cell lysate. Western Blot Analysis: Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Caspase 3 (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.07 M Tris-glycine,  pH 7.4, 0.105 M sodium chloride, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3 / Caspase 3

Background: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation