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Cyclin E1 antibody (C-Term)

CCNE1 Reactivity: Human WB, IHC, FACS, IP Host: Mouse Monoclonal HE-12 unconjugated

Catalog No. ABIN211140

Datasheet as PDF

Target See all Cyclin E1 (CCNE1) Antibodies

Cyclin E1 (CCNE1)
Binding Specificity
27

16

13

10

9

8

7

6

5

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
137

36

32

3

2

2

2
Human
Host
112

24

1
Mouse
Compatible Secondaries
Clonality
105

32
Monoclonal
Conjugate
77

7

5

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1
This Cyclin E1 antibody is un-conjugated
Application
90

42

41

38

28

28

19

13

10

8

3

3

3

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity

Specific for cyclin E. The epitope is located within the last 80 aa of the C-terminal. HE12 antibody detects a ~50kD band on human HeLa cell lysates by Western blot analysis. Reacts with human cyclin E (HeLa cells). Reactivity with other species has not been tested.

Purification

Protein A purified

Immunogen

Full-length, recombinant, human cyclin E protein.

Type of Immunogen: Recombinant protein

Clone

HE-12

Isotype

IgG1 kappa

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CCNE1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

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CCNE1 Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB07335 unconjugated

Application Notes

Approved: Flo, IHC, IP, WB

Usage: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Western Blot: 1 - 2 μg/mL. Immunoprecipitation: 1 μg/IP. Flow cytometry: Immunohistochemistry.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.2, 0.1 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
Target

Cyclin E1 (CCNE1)

Alternative Name

CCNE1 / Cyclin E1 (CCNE1 Products)

Background

Name/Gene ID: CCNE1

Synonyms: CCNE1, Cyclin E1, CCNE, Cyclin Es, Cyclin Et, G1/S-specific cyclin-E1, G1/s-specific cyclin e, Cyclin E

Gene ID

898

UniProt

P24864

Pathways

Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases