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Cyclin E1 antibody (AA 10-176)

CCNE1 Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal CCNE1-2460 unconjugated
Catalog No. ABIN6940994
  • Target See all Cyclin E1 (CCNE1) Antibodies
    Cyclin E1 (CCNE1)
    Binding Specificity
    • 28
    • 16
    • 15
    • 13
    • 8
    • 8
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 10-176
    Reactivity
    • 148
    • 38
    • 34
    • 3
    • 2
    • 2
    • 2
    Human
    Host
    • 125
    • 22
    • 1
    Mouse
    Clonality
    • 114
    • 34
    Monoclonal
    Conjugate
    • 83
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This Cyclin E1 antibody is un-conjugated
    Application
    • 104
    • 48
    • 47
    • 42
    • 36
    • 28
    • 22
    • 13
    • 13
    • 10
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)
    Clone
    CCNE1-2460
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product CCNE1 Primary Antibody
  • Application Notes

    Positive Control: Jurkat, MCF, A431, HeLa cells. Breast or Colon Carcinoma.

    Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Cyclin E1 (CCNE1)
    Alternative Name
    CCNE1 (CCNE1 Products)
    Synonyms
    CCNE1 antibody, ccne antibody, cyclinE antibody, CCNE antibody, AW538188 antibody, CycE1 antibody, CYCLE antibody, Ccne antibody, cyce2 antibody, cycE3 antibody, cyce antibody, fa18e07 antibody, wu:fa18e07 antibody, cyclin E1 antibody, cyclin E1 L homeolog antibody, cyclin E1 S homeolog antibody, CCNE1 antibody, ccne1 antibody, Ccne1 antibody, ccne1.L antibody, ccne1.S antibody
    Background
    Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
    Molecular Weight
    53kDa
    Gene ID
    898
    UniProt
    P24864
    Pathways
    Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
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