WNT2B antibody (Internal Region)

Catalog No. ABIN213587

The Rabbit Polyclonal anti-WNT2B antibody is suitable to detect WNT2B in samples from Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Pig, Monkey, Bat and Hamster. It has been validated for IHC and IHC (p).

Quick Overview for WNT2B antibody (Internal Region) (ABIN213587)

Target: WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Pig, Monkey, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT2B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-WNT2B Antibody

Binding Specificity: Internal Region

Specificity: Human WNT2B. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GALNT6 (56 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Panda, Horse, Rabbit, Pig (100%) Elephant (94%) Opossum, Platypus (88%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from internal region of human WNT2B. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Panda, Horse, Rabbit, Pig (100%), Elephant (94%), Opossum, Platypus (88%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for WNT2B

Target: WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))

Alternative Name: WNT2B

Background: Name/Gene ID: WNT2B
Family: WNT

Synonyms: WNT2B, Protein Wnt-2b, XWNT2, Protein Wnt-13, WNT13, XWNT2, Xenopus, homolog of

Gene ID: 7482

Pathways: WNT Signaling