NCS1 antibody

Catalog No. ABIN214036

This Rabbit Polyclonal antibody specifically detects NCS1 in WB, IHC, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for NCS1 antibody (ABIN214036)

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NCS1 Antibody

Specificity: Frequenin (recombinant from Mouse with extensive post-translational modifications).

Purification: Immunoaffinity purified

Immunogen: Recombinant mouse NCS1 / NCS-1.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Target Details for NCS1

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Alternative Name: NCS1 / Neuronal Calcium Sensor

Background: Name/Gene ID: NCS1

Synonyms: NCS1, Frequenin (Drosophila) homolog, FREQ, FLUP, Frequenin homolog, Neuronal calcium sensor 1, Frequenin homolog (Drosophila), Frequenin-like protein, NCS-1

Gene ID: 23413

UniProt: P62166