GLI3 antibody (AA 41-57)

Catalog No. ABIN214604

The Rabbit Polyclonal anti-GLI3 antibody is suitable to detect GLI3 in samples from Human, Chicken, Dog, Xenopus laevis, Quail and Squirrel Monkey. It has been validated for WB, IHC, IF, ELISA, ICC and IHC (p).

Quick Overview for GLI3 antibody (AA 41-57) (ABIN214604)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivity: Human, Chicken, Dog, Xenopus laevis, Quail, Squirrel Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GLI3 Antibody

Binding Specificity: AA 41-57

Specificity: Amino acids 41-57 of human Gli-3 protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human GLI3.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:6000 - 1:30000), ICC, IF, IHC, IHC-P (1.25 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1.25 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: GLI3

Background: Name/Gene ID: GLI3

Synonyms: GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3

Gene ID: 2737

UniProt: P10071

Pathways: Hedgehog Signaling