SCNN1B antibody (C-Term)

Catalog No. ABIN6736428

The Rabbit Polyclonal anti-SCNN1B antibody is suitable to detect SCNN1B in samples from Human, Mouse, Rat, Cow, Goat, Monkey and Bat. It has been validated for WB, IHC and IHC (p).

Quick Overview for SCNN1B antibody (C-Term) (ABIN6736428)

Target: SCNN1B (Sodium Channel, Nonvoltage-Gated 1, beta (SCNN1B))

Reactivity: Human, Mouse, Rat, Cow, Goat, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCNN1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SCNN1B Antibody

Binding Specificity: C-Term

Specificity: Human SCNN1B

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Goat (100%) Horse, Guinea pig (92%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SCNN1B (P51168, NP_000327). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Galago, Goat, Elephant, Bovine, Bat, Opossum (100%), Gorilla, Rabbit, Horse, Guinea pig, Platypus (92%), Mouse, Rat, Hamster (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.12 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SCNN1B

Target: SCNN1B (Sodium Channel, Nonvoltage-Gated 1, beta (SCNN1B))

Alternative Name: SCNN1B / ENaC Beta

Background: Name/Gene ID: SCNN1B
Subfamily: Sodium channel - amiloride-sensitive
Family: Ion Channel

Synonyms: SCNN1B, BESC1, Beta-NaCH, Beta ENaC, Beta-ENaC, ENaC beta, ENaCb, HENaC, Liddle syndrome, RENaC, RNENACB, SCNEB, ENaCbeta

Gene ID: 6338

NCBI Accession: NP_000327

UniProt: P51168