IL-3 antibody

Catalog No. ABIN218090

The Rat Monoclonal anti-IL-3 antibody is suitable to detect IL-3 in samples from Human. It has been validated for WB, ELISA and Neut.

Quick Overview for IL-3 antibody (ABIN218090)

Target: IL-3 (Interleukin 3 (IL-3))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-3 Antibody

Specificity: Recognizes human Interleukin-3 (IL-3).

Purification: Purified

Immunogen: Yeast-expressed recombinant human IL-3.

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Neut, WB

Usage: ELISA Capture: Purified monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human IL-3 protein levels.1-3 The purified antibody should be paired with biotinylated antibody as the detection antibody, with purified recombinant human IL-3 as the standard. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-3 under reducing conditions. Neutralization: The LE/AF form of this antibody can be used for neutralizing human IL-3 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS. No preservative added.

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL3

Background: Name/Gene ID: IL3
Family: Hormone

Synonyms: IL3, Hematopoietic growth factor, IL-3, Mast-cell growth factor, MULTI-CSF, P-cell-stimulating factor, Interleukin-3, Mast cell growth factor, MCGF, P-cell stimulating factor

Gene ID: 3562

UniProt: P08700

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy