BCAR1 antibody

Catalog No. ABIN221802

The Mouse Monoclonal anti-BCAR1 antibody (ABIN221802) specifically detects BCAR1 in WB, IHC, IHC (p) and IP. The antibody is reactive with Human and Rat samples.

Quick Overview for BCAR1 antibody (ABIN221802)

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-BCAR1 Antibody

Specificity: Recognizes p130Cas. Species cross-reactivity: human and rat.

Purification: Protein G purified

Immunogen: Recombinant human p130cas protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-P, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Denatured verified): 2 μg/mg protein lysate. (Use Protein G). Western Blot:1-2 μg/mL for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1-2 μg/mL for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: MCF-7 cells, HUVEC cells. Tonsil or breast carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: BCAR1 / p130Cas

Background: Name/Gene ID: BCAR1

Synonyms: BCAR1, CAS, CAS1, CRKAS, CASS1, CRK-associated substrate, p130Cas

Gene ID: 9564

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling