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Cyclin E1 antibody (pThr62)

CCNE1 Reactivity: Human WB, IHC, FACS, IHC (p), IP Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN227700

Datasheet as PDF

Target See all Cyclin E1 (CCNE1) Antibodies

Cyclin E1 (CCNE1)
Binding Specificity
27

16

13

11

9

8

7

6

5

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr62
Reactivity
137

36

32

3

2

2

2
Human
Host
111

25

1
Rabbit
Compatible Secondaries
Clonality
104

33
Polyclonal
Conjugate
77

7

5

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1
This Cyclin E1 antibody is un-conjugated
Application
90

42

41

38

28

28

18

13

10

8

3

3

3

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity

Detects endogenous levels of cyclin E only when phosphorylated at threonine 62 (cyclin E1 isoform 2) or threonine 77 (cyclin E1 isoform 1). Species cross-reactivity: Human.

Purification

Immunoaffinity purified

Immunogen

Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr62 of human cyclin E.

Type of Immunogen: Synthetic phospho-peptide

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CCNE1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

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Application Notes

Approved: Flo (1:25), IHC, IHC-P (1:100), IP (1:100), WB (1:1000)

Usage: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Flow Cytometry: 1:25. Western Blot: 1:1000. Immunohistochemistry: (Paraffin) 1:100. Citrate/TBST. Immunoprecipitation: 1:100.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.

Handling Advice

Avoid repeat freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

Cyclin E1 (CCNE1)

Alternative Name

CCNE1 / Cyclin E1 (CCNE1 Products)

Background

Name/Gene ID: CCNE1

Synonyms: CCNE1, Cyclin E1, CCNE, Cyclin Es, Cyclin Et, G1/S-specific cyclin-E1, G1/s-specific cyclin e, Cyclin E

Gene ID

898

UniProt

P24864

Pathways

Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases