Hepatitis Virus (Strain A59) Nonstructural Protein 9 (Protein A Purified) (nsp9) antibody

Antigen: Hepatitis Virus (Strain A59) Nonstructural Protein 9 (Protein A Purified) (nsp9)

Clonality: Monoclonal

Host: Mouse

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233751

Additional Information

Alternative name: Hepatitis Virus (Strain A59) Nonstructural Protein 9 (nsp9) (Protein A Purified)

Gene ID: NP_068668 MHV-A59

Immunogen: This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.

Format: Liquid (sterile filtered)

Description: The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.

Specificity: This antibody is directed against the MHV-A59 nsp9 protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologues from other sources has not been tested.

Application Details

Application Notes: This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. Note: This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. Dilutions: WESTERN BLOT 1:1,000 IF MICROSCOPY 1:1,000 IMMUNO PRECIPITATION User Optimized OTHER APPLICATIONS User Optimized

Concentration: 2.1 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blotting using antibodies-online Immunochemicals anti-MHV-A59 nsp9 antibody to detect protein in various lysates, 6h post MHV infection. Lane 1 shows no crossreactivity with SARS-CoV-infected Vero cells. Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown in lane 3. Negative controls (lanes 2 and 4) show no staining. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.

Immunofluorescence microscopy using antibodies-online Immunochemical's anti-MHV-A59 nsp9 antibody, 6-h post infection in mouse L cells. Cells were fixed in 3% paraformaldehyde. For detection Cy2 conjugated Goat-anti- Mouse IgG MX10 (610-111-121) was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.