EGFR antibody (pSer1047)

Catalog No. ABIN235074

Product Details anti-EGFR Antibody

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Binding Specificity: pSer1047

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Indirect Immunofluorescence Assay (IFA)

Cross-Reactivity (Details): The EGF Receptor (EFR) is activated upon stimulation with growth factors of the EGF family and autophosphorylates at multiple tyrosine residues. Serine phosphorylation of the carboxy terminal domain at Serine 1047 leads to ligand-dependent EGFR desensitization. However, if serine 1047 is phosphorylated, the neighboring tyrosine 1045 may not be phosphorylated due to sterical hindrance, thus inhibiting cbl-mediated receptor degradation. Clone 1H9 specifically recognizes EGFR phosphorylated at Serine 1047. Reacts with Human, mouse and dog.

Characteristics: Cell lysate of 2 x 10^6 vanadate treated HepG2 cells.

Purification: Thiophilic adsorption and size exclusion Chromatography. Cell culture

Immunogen: Synthetic phosphopeptide corresponding to AA residues surrounding Serine 1047

Clone: 1H9

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 µg/mL for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5 % (w/v) casein, 5 % (w/v) PEG 4000, 5 % (w/v) Polyvinylpyrrolidone (PVP), 0.5 % Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. EGFR Transactivation Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE (20.000 cell per lane). The blot was probed with 0.5- g/mL K67190M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 5: Sorbit Lane 2: PMA Lane 6: Anisomycin Lane 3: Forskolin Lane 7: Lonomycin Lane 4: LPA Lane 8: Taxol

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL water for 15 minutes at room temperature.

Buffer: Lyophilized from PBS, PEG, and Sucrose, 0.09 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Store lyophilized product at -20 °C. After reconstitution, aliquot and store at -80 °C. Thawed aliquots may be stored at 2-0 °C for up to 3 months.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Abstract: EGFR Products

Synonyms: C-erb antibody, CG10079 antibody, D-EGFR antibody, D-Egf antibody, DEGFR antibody, DER antibody, DER flb antibody, DER/EGFR antibody, DER/faint little ball antibody, DER/top antibody, DER/torpedo antibody, DER1 antibody, DEgfr antibody, Degfr antibody, Der antibody, DmHD-33 antibody, Dmel\\CG10079 antibody, EFG-R antibody, EGF-R antibody, EGFR antibody, EGFr antibody, EGfr antibody, EK2-6 antibody, Egf antibody, Egf-r antibody, EgfR antibody, El antibody, Elp antibody, Elp-1 antibody, Elp-B1 antibody, Elp-B1RB1 antibody, HD-33 antibody, TOP antibody, Torpedo/DER antibody, Torpedo/Egfr antibody, c-erbB antibody, d-egf-r antibody, dEGFR antibody, dEGFR1 antibody, dEgfr antibody, der antibody, egfr antibody, flb antibody, l(2)05351 antibody, l(2)09261 antibody, l(2)57DEFa antibody, l(2)57EFa antibody, l(2)57Ea antibody, mor1 antibody, top antibody, top/DER antibody, top/flb antibody, torpedo/Egfr antibody, torpedo/egfr antibody, EGFR12 antibody, EGFR15 antibody, egfr1 antibody, Erbb2 antibody, ERBB antibody, ERBB1 antibody, HER1 antibody, PIG61 antibody, mENA antibody, ErbB-1 antibody, Errp antibody, 9030024J15Rik antibody, AI552599 antibody, Erbb antibody, Errb1 antibody, Wa5 antibody, wa-2 antibody, wa2 antibody, epidermal growth factor receptor antibody, Epidermal growth factor receptor antibody, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) antibody, EGFR antibody, Egfr antibody, egfra antibody, egfr1 antibody, LOC5564544 antibody

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins