Collagen Type V antibody

Catalog No. ABIN237044

Product Details anti-Collagen Type V Antibody

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type V antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV, and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Characteristics: Rabbit anti Collagen Type V, Rabbit antibody to Human Collagen Type V

Purification: Immuno Affinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Immunogen: Collagen Type V from human and bovine placenta

Application Details

Application Notes: Have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve and for non-denaturing immunoblot for highly sensitive qualitative analysis. Was assayed by non-denaturing immunoblot and found to be reactive against Collagen V at a dilution of 1:5.000 to 1:10.000. This product was also assayed against 1.0 µg of Collagen V in a standard ELISA using Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG (H&L) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:8.000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C

Storage Comment: Upon receipt, store at 2-8 °C as an undiluted liquid. For long term storage, mix with an equal volume of glycerol, aliquot and store at -28 °C.

Expiry Date: 12 months

Target Details for Collagen Type V

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Alternative Name: Collagen Type V (COL5 Products)