Collagen Type V antibody

Catalog No. ABIN135054

Cited in 3+ publications. The Goat Polyclonal anti-Collagen Type V antibody (ABIN135054) specifically detects Collagen Type V in WB, ELISA, IHC (fro), IHC (p), ICC and IEM. The antibody is reactive with Human, Rat, Mouse, Dog, Chicken, Guinea Pig, Horse, Pig, Rabbit, Sepiidae and Tree shrew samples.

Quick Overview for Collagen Type V antibody (ABIN135054)

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Reactivity: Human, Rat, Mouse, Dog, Chicken, Guinea Pig, Horse, Pig, Rabbit, Sepiidae, Tree shrew

Host: Goat

Clonality: Polyclonal

Conjugate: This Collagen Type V antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Product Details anti-Collagen Type V Antibody

Purpose: Goat Anti-Type V Collagen-UNLB

Specificity: Reacts with conformational determinants on type V collagen Referenced species reactivities include - Human 2,5,6,11,13,14,17,23-26 Chicken 4 Porcine 1,5,8 Mouse 3,12,20 Cuttlefish 27 Rabbit 9,15 Rat 5,7,10,16 Equine 19,21,22 Tree Shrew 18 Canine 5 Guinea Pig 15

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Purified Anti-Type V Collagen antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, and western blotting assays.

Purification: Purified by affinity chromatography on type V collagen covalently linked to agarose, cross-adsorbed against Collagen types I, II, III, IV, and VI for minimal reactivity

Immunogen: Type V collagen

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with type V collagen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: Borate buffered saline, pH 8.2

Storage: 4 °C

Storage Comment: 2-8°C

References for anti-Collagen Type V antibody (ABIN135054)

Welc, Flores, Wehling-Henricks, Ramos, Wang, Bertoni, Tidball: "Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy." in: Nature communications, Vol. 10, Issue 1, pp. 2788, (2019) (PubMed).

Finno, Gianino, Perumbakkam, Williams, Bordbari, Gardner, Burns, Peng, Durward-Akhurst, Valberg: "A missense mutation in MYH1 is associated with susceptibility to immune-mediated myositis in Quarter Horses." in: Skeletal muscle, Vol. 8, Issue 1, pp. 7, (2018) (PubMed).

Huang, Ge, Izzi, Greenspan: "α3 Chains of type V collagen regulate breast tumour growth via glypican-1." in: Nature communications, Vol. 8, pp. 14351, (2018) (PubMed).

Target Details for Collagen Type V

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Alternative Name: TypeV Collagen

Background:

Description: Goat Anti-Type V Collagen-UNLB

NCBI_Official_Symbol: COL5A1, COL5A2, COL5A3

Synoynms: COL5

Gene ID: 1289, 1290

UniProt: P20908