Kinesin Family Member 5B (KIF5B) antibody

Antigen: Kinesin Family Member 5B (KIF5B)

Synonyms: KNS, KINH, KNS1, UKHC, Khc, KIF5A, DKFZp459M0928

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN238341

Additional Information

Characteristics: Synonyms: KIF5B, KNS, KNS1, Kinesin-1 heavy chain, Ubiquitous kinesin heavy chain, UKHC,Conventional kinesin heavy chain

Alternative name: KIF5B

Swiss-Prot: P33176

Immunogen: stalk domain of human kinesin (aa 331-906) expressed in E. coli (FKHC3)

Cross-Reactivity: Human, Mouse (Murine), Pig (Porcine)

Description: Kinesin belongs to the group of microtubule-associated motor proteins known to convertchemical energy released from nucleoside triphosphates (preferentially from ATP) intomechanical energy. Conventional kinesin, member of the kinesin superfamily comprisingmore than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of twoheavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular massabout 400 kDa. Each heavy chain contains an N-terminal globular motordomain with botha microtubule-binding site and an ATPase active center, stalk region responsible for heavychain dimerization and finally C-terminal globular tail domain, which is implicated in cargobinding. Light chains may have a regulatory function.

Specificity: The polyclonal antibody detects total level of endogenous kinesin protein. Species: Human, Porcine, Mouse. Others not tested.

Application Details

Application Notes: Western Blotting. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cytoskeleton

Restrictions: For Research Use only