Early Secretory Antigenic Target ESXA (ESAT-6) antibody

Antigen: Early Secretory Antigenic Target ESXA (ESAT-6)

Clonality: Polyclonal

Host: Rabbit

Application: Western Blotting (WB)

Catalog no.: ABIN238348

Additional Information

Alternative name: ESAT6

Gene ID: 886209

Swiss-Prot: P0A564

Immunogen: EsaT-6 (Rv3875) of Mycobacterium tuberculosis.

Description: The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediatedimmunity in the early phase of tuberculosis (TB) in TB patients as well as in various animalmodels. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits highlevel of IFN-gamma from memory effector cells during the first phase of a protectiveimmune response.

Specificity: The polyclonal antibody reacts with EsaT-6, a 11,8 kDa antigen encoded by gene Rv3875 ofMycobacterium tuberculosis. The antibody recognizes EsaT-6 of Mycobacterium tuberculosis. H37Rv and Mycobacteriumbovis. It reacts also with recombinant antigen produced in Escherichia coli.

Application Details

Application Notes: Suitable for Western Blotting under Reducing conditions: 0.2-1 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Pictures:

Research Area: Bacteria, Gram positive bacteria

Restrictions: For Research Use only