CD4 antibody

Catalog No. ABIN238364

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS, WB, IHC (fro), Func and EIA. It is suitable to detect CD4 in samples from Human.

Quick Overview for CD4 antibody (ABIN238364)

Target: CD4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Clone: 13B8-2

Product Details anti-CD4 Antibody

Specificity: The CD4 antigen is a monomeric transmembrane glycoprotein with a molecular weight (Mr) of 60 kDa. CD4 Molecule is expressed on a specific subset of peripheral blood T lymphocytes named ''helper/inducer''. The CD4 antige is present on approximately 45 % of peripheral blood lymphocytes (T4 lymphocytes), 80 % of thymocytes and with a lower density of 100 % of monocytes and on some neutrophils.

Characteristics: Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

Immunogen: Human thymocytes

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Studies of CD4 expressing cells. Fluorescence microscopy or Flow cytometry: 2 g/5x10e5 cells/test. Immunohistochemistry: Working dilution 1/25-1/50. Only suitable on cryostat sections or cell smears.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 1 mL distilled water.

Concentration: 0.2 mg/mL (prior to lyophilization)

Buffer: PBS containing 1 mg/mL BSA as stabilizer without preservatives.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at-20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID: 920

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events