Lck Interacting Transmembrane Adaptor 1 (LIME1) antibody (PE)

Antigen: Lck Interacting Transmembrane Adaptor 1 (LIME1)

Synonyms: LIME, LP8067, FLJ20406, dJ583P15.4, RP4-583P15.5, 2810038K19Rik, RGD1562795, LIME1, DKFZp459A158

Clonality: Monoclonal (LIME06)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN238427

Additional Information

Alternative name: LIME1

Swiss-Prot: Q9H400

Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME

Cross-Reactivity: Human

Isotype: IgG1

Clone: LIME06

Description: LIME (Lck-interacting molecule) is a 31 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative- regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals. Alternate Names: Lck-Interacting Molecule.

Specificity: The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 31 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Application Details

Application Notes: Suitable for Flow Cytometry analysis (5 ug/ml). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.

Storage: Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf Life: one year from despatch.

Research Area: Signaling

Restrictions: For Research Use only