Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibody

Details for Product No. ABIN94499
Antigen
Epitope
AA 141-295
20
18
11
10
7
6
5
1
1
Reactivity
Human
64
18
Host
Rabbit
38
36
7
Clonality
Polyclonal
Conjugate
Un-conjugated
8
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
68
21
21
11
11
2
Options
Immunogen Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
Specificity The polyclonal antibody reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Human
Purification Purified from rabbit serum by precipitation methods
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name LIME (LIME1 Antibody Abstract)
Background LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibody (ABIN94499) Immunohistochemistry staining of human spleen (paraffin sections) with anti-LIME poly...
Product cited in: Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).