CD95 antibody
| Antigen |
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| Clonality |
Monoclonal (LT95) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (63), Western Blotting (WB) (34), Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Blocking Antibody (Inhibition) (8), ELISA (6), Immunofluorescence (IF) (5), ELISA (Detection) (3), Dot Blot (Dot) (2), Immunohistochemistry (IHC) (2)
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| Catalog no. |
ABIN238438 |
| Quantity |
0.1 mg (1.0 mg/ml) |
| Price |
265.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptorsuperfamily member 6
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Alternative name
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CD95 / FAS
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Swiss-Prot
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P25445
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Immunogen
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HUT-78 human T cell lymphoma cell line
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Cross-Reactivity
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Human
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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LT95
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Description
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CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of theTNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular deathdomains, formation of the death-inducing signaling complex (DISC) and activation ofcaspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated atthe DISC, in type II cells low concentration of caspase 8 activates pathway leading to therelease of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokineresponses.
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Specificity
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The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoproteinof the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily,expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although itcross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule. Species: HumanOthers not tested.
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Application Notes
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Flow Cytometry. Immunohistochemistry (paraffin sections). Positive tissue: tonsilOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Purified
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Buffer
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20 mM Tris/HCl, pH 7.4 with 15 mM sodium azide as preservative.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD95", type "Antibodies"
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Hosts
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Mouse (91), Rabbit (20), Goat (1)
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Reactivities
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Human (109), Monkey (3), Mouse (Murine) (3), Rat (Rattus) (3)
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Applications
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Flow Cytometry (FACS) (63), Western Blotting (WB) (34), Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Blocking Antibody (Inhibition) (8), ELISA (6), Immunofluorescence (IF) (5), ELISA (Detection) (3), Dot Blot (Dot) (2), Immunohistochemistry (IHC) (2)
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Conjugates
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FITC (10), PE (10), APC (6), Biotin (4), SPRD (2)
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Epitopes
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C-Term (4), pTyr232 (2), pTyr291 (2), Center (1)
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