CD95 antibody (PE)
| Antigen |
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| Clonality |
Monoclonal (LT95) |
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Host
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Reactivity
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Conjugate
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Application
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Flow Cytometry (FACS) (63), Western Blotting (WB) (34), Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Blocking Antibody (Inhibition) (8), ELISA (6), Immunofluorescence (IF) (5), ELISA (Detection) (3), Dot Blot (Dot) (2), Immunohistochemistry (IHC) (2)
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| Catalog no. |
ABIN238439 |
| Quantity |
100 Tests |
| Price |
330.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptorsuperfamily member 6
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Alternative name
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CD95 / FAS
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Swiss-Prot
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P25445
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Immunogen
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HUT-78 human T cell lymphoma cell line.
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Cross-Reactivity
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Human
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Isotype
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IgG1
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Clone
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LT95
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Description
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CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of theTNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular deathdomains, formation of the death-inducing signaling complex (DISC) and activation ofcaspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated atthe DISC, in type II cells low concentration of caspase 8 activates pathway leading to therelease of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokineresponses.
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Specificity
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The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoproteinof the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily,expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although itcross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule. Species: Human. Others not tested.
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Application Notes
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Suitable for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl ofwhole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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PBS containing 15 mM sodium azide as preservative and 0.2% (w/v)high-grade BSA (Protease free) as stabilizer.Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions.
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Storage
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Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD95", type "Antibodies"
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Hosts
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Mouse (91), Rabbit (20), Goat (1)
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Reactivities
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Human (109), Monkey (3), Mouse (Murine) (3), Rat (Rattus) (3)
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Applications
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Flow Cytometry (FACS) (63), Western Blotting (WB) (34), Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Blocking Antibody (Inhibition) (8), ELISA (6), Immunofluorescence (IF) (5), ELISA (Detection) (3), Dot Blot (Dot) (2), Immunohistochemistry (IHC) (2)
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Conjugates
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FITC (10), PE (9), APC (6), Biotin (4), SPRD (2)
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Epitopes
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C-Term (4), pTyr232 (2), pTyr291 (2), Center (1)
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Alternatives 
