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Antibodies
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anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody (PE)
Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody (PE)
| Antigen |
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| Synonyms |
CD31, PECAM-1, FLJ58394, Cd31, Pecam, C85791, MGC102160, MGC124998, PECAM1 |
| Clonality |
Monoclonal (MEM-05) |
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Host
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Reactivity
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Human (156), Mouse (Murine) (69), Rat (Rattus) (54), Dog (Canine) (35), Pig (Porcine) (33), Horse (Equine) (18), Rabbit (17), Cow (Bovine) (7), Sheep (Ovine) (6), Monkey (5), Chicken (2)
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Conjugate
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FITC (21), Biotin (14), PE (12), APC (6), PE,Cy7 (4), Alexa Fluor 488 (3), RPE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), RBITC (2), Alexa Fluor 700 (1), PE,DY590 (1), PE-Dyomics 590 (1), Pacific Blue (1), PerCP (1)
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Application
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Flow Cytometry (FACS) (149), Western Blotting (WB) (67), Immunofluorescence (IF) (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (34), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunohistochemistry (IHC) (22), Immunoprecipitation (IP) (22), ELISA (16), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), ELISA (Detection) (3), Functional Studies (Func) (3), Blocking Antibody (Inhibition) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (EM) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN238443 |
| Quantity |
100 Tests |
| Price |
330.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: PECAM-1, EndoCAM, GPIIA', Platelet endothelial cell adhesion molecule
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Alternative name
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CD31 / PECAM1
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Swiss-Prot
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P16284
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Immunogen
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Leukocytes of patient suffering from LGL-type leukaemia
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Cross-Reactivity
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Human
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Isotype
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IgG1
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Clone
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MEM-05
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Description
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CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptorinvolved in rebulation of T cell and B cell signaling by a dual immunoreceptortyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediatedphosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly atjunctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for itshomophilic binding, which plays an important role in cell-cell interactions. CD31 is amultifunctional molecule with diverse roles in modulation of integrin-mediated celladhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation ofimmunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediatedanaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
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Specificity
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The antibody MEM-05 reacts with CD31 (PECAM-1), a 130-140 kDa type I transmembraneglycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stemcells of the myeloid lineage. Species: Human. Others not tested.
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Application Notes
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Suitable for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl ofwhole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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PBS containing 15 mM sodium azide as preservative and 0.2 % (w/v)high-grade BSA (Protease free) as stabilizer.Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions
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Storage
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Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf life: One year from despatch.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "Platelet/endothelial Cell Adhesion Molecule (PECAM1)", type "Antibodies"
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Hosts
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Mouse (125), Rabbit (61), Rat (17), Hamster (2), Sheep (1)
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Reactivities
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Human (156), Mouse (Murine) (69), Rat (Rattus) (54), Dog (Canine) (35), Pig (Porcine) (33), Horse (Equine) (18), Rabbit (17), Cow (Bovine) (7), Sheep (Ovine) (6), Monkey (5), Chicken (2)
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Applications
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Flow Cytometry (FACS) (149), Western Blotting (WB) (67), Immunofluorescence (IF) (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (34), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunohistochemistry (IHC) (22), Immunoprecipitation (IP) (22), ELISA (16), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), ELISA (Detection) (3), Functional Studies (Func) (3), Blocking Antibody (Inhibition) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (EM) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (21), Biotin (14), PE (12), APC (6), PE,Cy7 (4), Alexa Fluor 488 (3), RPE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), RBITC (2), Alexa Fluor 700 (1), PE,DY590 (1), PE-Dyomics 590 (1), Pacific Blue (1), PerCP (1)
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Epitopes
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C-Term (13), pTyr713 (1)
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Alternatives 
