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Antibodies
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anti-Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) antibody
Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) antibody
| Antigen |
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| Synonyms |
CD16, FCG3, CD16A, FCGR3, IGFR3, FCR-10, FCRIII, FCGRIII, FCRIIIA |
| Clonality |
Monoclonal (MEM-168) |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (117), Immunoprecipitation (IP) (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Functional Studies (Func) (12), Western Blotting (WB) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunocytochemistry (ICC) (5), ELISA (3), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (2), Blocking Antibody (Inhibition) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN238452 |
| Quantity |
0.1 mg (1.0 mg/ml) |
| Price |
265.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: IgG Fc receptor III-2, Fc-gamma RIII-alpha, FcR-10, CD16a, FCGR3A, FCG3, IGFR3, Fc-gammaRIIIa, FcRIIIa, Fc-gamma RIII, Low affinity immunoglobulin gamma Fc region receptor III-A
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Alternative name
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CD16
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Swiss-Prot
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P08637
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Immunogen
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Human granulocytes.
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Cross-Reactivity
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Human
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Isotype
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IgM
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Clone
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MEM-168
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Description
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CD16 (FcγRIII) is a 50-80 kDa glycoprotein serving as a low affinity IgG receptor. HumanFcγRIII is expressed in two forms FcγRIII-A and -B. FcγRIII-A is a transmembrane protein ofmonocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γsubunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIII-Ais associated, moreover, with FcεRI-β subunit. Besides IgG, FcεRI-A can be triggered alsoby oligomeric IgE. FcγRIII-B is a GPI-linked monomeric receptor expressed on neutrophilsand is involved in their activation and induction of a proadhesive phenotype.
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Specificity
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The antibody MEM-168 reacts with CD16 antigen (isoform CD16a). CD16a (FcγRIIIA, 50-65kDa) is a transmembrane form of CD16, expressed on NK-cells, monocytes andmacrophages. Species: Human, Non-Human Primates, Porcine. Others not tested.
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Application Notes
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Suitable for Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Purified
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Buffer
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PBS with 15 mM sodium azide as preservative.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A)", type "Antibodies"
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Hosts
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Mouse (120), Rat (6), Rabbit (1)
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Reactivities
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Human (112), Pig (Porcine) (10), Primates (9), Mouse (Murine) (3), Monkey (2), Primate (1)
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Applications
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Flow Cytometry (FACS) (117), Immunoprecipitation (IP) (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Functional Studies (Func) (12), Western Blotting (WB) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunocytochemistry (ICC) (5), ELISA (3), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (2), Blocking Antibody (Inhibition) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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FITC (17), Biotin (14), PE (14), RPE (4), Alexa Fluor 700 (2), Cy5.5,PE (2), SPRD (2), Alexa Fluor 488 (1), PE,Cy5 (1), PE-Dyomics 647 (1), Pacific Blue (1), PerCP (1), RPE,Cy5 (1)
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Alternatives 
